Comparison of LRRK2-mediated phosphorylation of Rab10 in neutrophils and PBMCs.
(A) Both neutrophils and PBMCs were prepared from the same six healthy donors and treated with or without 100 nM MLi-2 for 30 min. Cells were then lysed and 10 µg of whole cell extract subjected to quantitative immunoblot analysis with the indicated antibodies (all at 1 µg/ml antibody) and the membranes were developed using the Odyssey CLx scan Western Blot imaging system. (B) Quantitation of immunoblots was undertaken analysing total LRRK2 (full length)/GAPDH (Bi), total Rab10/GAPDH ratio (Bii), phospho-Ser935 LRRK2 (full length)/total LRRK2 (full length) (Biii) and total phospho-Thr73 Rab10/total Rab10 (Biv).