Figure 2.
![HEK293 cell extracts overexpressing LRRK2[Y1699C] and the indicated HA-tagged Rab protein were treated ±150 nM MLi-2 for 90 min and then lysed. The cell lysates were immunoblotted with the indicated sheep (A) and rabbit (B) affinity-purified phospho-specific polyclonal antibodies (all at 1 µg/ml antibody — for only sheep polyclonal antibodies, we also included 10 µg/ml dephospho-peptide antigen). Ten micrograms of whole cell lysate were loaded per lane for the sheep polyclonal antibodies (A) and 1 µg of cell lysate per lane for the rabbit polyclonal antibodies (B). Immunoblots developed with ECL.](https://port.silverchair-cdn.com/port/content_public/journal/biochemj/475/1/10.1042_bcj20170802/2/bcj-2017-0802_02.jpeg?Expires=1716992150&Signature=OgxdhiHzIokfLfh-EVLfqr~tizdFTma3CzqlGi2sE36AYkobGXn0~hwKYSv6-0ZvfSeEgO~drTNSWYPOdRw2GEZ0Ar4ccSJmrrFvsAKbi8sREn-zxPNzOLBWSu6LEVdS649YFDc4X760IMqpfGNqtagQTSNoLfWELvH21kW1dQ9rcjMVhzYxaa68sfSXQ4gH1kzKc9JhDr50yhdXnQWUivSCqPEgFD3YHhuSA1wYBuHzzs1J8CMej11SwE1bk1~n8PozIoWEWJ1iya0VoPMC~iTCP4Js65dtcGo4~AbBvjhzhxueS5DN974lUVGod2vt4j86kb2CQ0RultMXO-WapQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cross-reactivity of the polyclonal sheep and rabbit phospho-Rab protein antibodies.
HEK293 cell extracts overexpressing LRRK2[Y1699C] and the indicated HA-tagged Rab protein were treated ±150 nM MLi-2 for 90 min and then lysed. The cell lysates were immunoblotted with the indicated sheep (A) and rabbit (B) affinity-purified phospho-specific polyclonal antibodies (all at 1 µg/ml antibody — for only sheep polyclonal antibodies, we also included 10 µg/ml dephospho-peptide antigen). Ten micrograms of whole cell lysate were loaded per lane for the sheep polyclonal antibodies (A) and 1 µg of cell lysate per lane for the rabbit polyclonal antibodies (B). Immunoblots developed with ECL.
