(A) Effect of bosutinib and dasatinib on cytokine secretion. Bone-marrow-derived macrophages were treated with vehicle control, 3 μM bosutinib or 0.3 μM dasatinib for 1 h and then stimulated with LPS for 8 h. The concentration of the different cytokines in the culture supernatant was measured using the BIOPLEX system. The data are depicted as the fold-change in cytokine secretion in the presence of the drug (n=4, mean±S.E.M.). Statistical significance was determined by comparing each dataset to 0 using a one-sample Student's t test. (B) Effect of bosutinib and dasatinib on the expression of markers of ‘regulatory’ macrophages. Bone-marrow-derived macrophages were treated with vehicle control, 3 μM bosutinib or 0.3 μM dasatinib for 1 h and then stimulated with LPS for the indicated times. Expression of LIGHT, SPHK1 and arginase 1 were measured by qPCR. mRNA levels were normalized to 1 in unstimulated cells (mean±S.E.M., n=4). (C) Effect of bosutinib and dasatinib on the expression of markers of alternatively activated macrophages. Experiment was performed as described in (B) but measuring the mRNA levels of FIZZ, Ym1 and Mgl2 (mean±S.E.M., n=4).