Bosutinib and dasatinib induce the dephosphorylation of CRTC3 and its nuclear translocation
(A) RAW264.7 macrophages were treated with bosutinib, dasatinib, HG-9-91-01 or vehicle control for 1 h. CRTC3 was immunoprecipitated and phosphorylation of Ser162 and Ser370 was monitored by immunoblotting. (B) Bone-marrow-derived macrophages were plated on glass coverslips, and treated with bosutinib, dasatinib, HG-9-91-01 or vehicle control for 1 h. Samples were stained for CRTC3 (Alexa Fluor® 594), tubulin (Alexa Fluor® 488) and DNA (DAPI) content and imaged under a confocal microscope. (C) Quantification of CRTC3 nuclear translocation as a function of its nuclear to cytoplasmic ratio. (mean±S.E.M., n=10).