Figure 1
![(A) Strategies 1, 2 and 3 for making orthogonal CaM (orange)–M13 (grey) pairs with side chains of R-GECO1 (PDB code 4I2Y) shown in stick format [8]. (B) Normalized fluorescence intensities of LAR-GECO1, LAR-GECO1.2 and R-GECO1 as a function of free Ca2+ concentrations in buffer (10 mM Mops and 100 mM KCl, pH 7.2). The black line for R-GECO1 is a curve fitted to data reported previously [5], with Kd=482 nM and Hill slope=2.06.](https://port.silverchair-cdn.com/port/content_public/journal/biochemj/464/1/10.1042_bj20140931/2/bj4640013fig1.jpeg?Expires=1716622873&Signature=gcCM14~Jq6sd43wQ2XXZXMf6-AkBInW6ha5w4fq8OYCtycWS06P8qbC1yxNPmw27IeAt1BM5EaL8LGk9~qZl3svfuqmd-A7-oZjOhnbTe7Z44q4l9Yjy23-meco0n4oD5seNGRVW4kvnVwQSf3NSHHfVIPZXHgs5c6R9nmYbRMh9YYX46lAFkUPPBzgeAIVkb6XQ8YPJJd90I9g6qO-9SY37fi~CL16cmmXYajmiH50VBO1yBAcAaX5xmhG3HmvnMVM0seb0DBRnBw~cLPosOvpX8nBl8QJjCdbXgJczxIZ7rUj0fMBMiyFMxD3H6gJQGWmXyX~CGmXMEF34IOuKPg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Strategies for development of LAR-GECO1 and LAR-GECO1.2 and their dissociation constants
(A) Strategies 1, 2 and 3 for making orthogonal CaM (orange)–M13 (grey) pairs with side chains of R-GECO1 (PDB code 4I2Y) shown in stick format [8]. (B) Normalized fluorescence intensities of LAR-GECO1, LAR-GECO1.2 and R-GECO1 as a function of free Ca2+ concentrations in buffer (10 mM Mops and 100 mM KCl, pH 7.2). The black line for R-GECO1 is a curve fitted to data reported previously [5], with Kd=482 nM and Hill slope=2.06.
