2D resolution of the mitochondrial complexes in S. renifolius (A) and A. maculatum (B) after separation by 2D BN/SDS/PAGE
Mitochondrial proteins were solubilized by digitonin (5 g/g of protein) prior to 1D BN-PAGE, and then reduced and alkylated prior to 2D SDS-PAGE. The proteins were stained using Coomassie Brilliant Blue. Molecular masses of standard proteins in the second dimension are indicated to the right-hand side of the gels. The identities of the protein complexes specified in Figure 1 are indicated above the gels. Arrows mark the proteins identified by MS. Numbers and letters attached to the arrows correspond to those used for the respective protein identities listed in Table 1. The contrast and brightness of the original images were manually adjusted for maximum clarity.