Expression from tagged and untagged PIP5K2A and PIP5K2B alleles determined by qRT-PCR using the comparative threshold cycle (C_t) method. Normalized expression is presented as the relative fold increase above untagged PIP5K2B in WT cells (n=9). PCR was performed on cDNA made from RNA extracts of WT, JPR3 and MW2 cells as described in the Materials and methods section.