(A) Control untransfected and COS-hTRPC3-1 cells were transfected with a construct that expresses a soluble secreted form of alkaline phosphatase. At 24 h post-transfection, they were cultured in the presence or absence of 50 μM ZnCl2 for a further 48 h and assayed in triplicate for SEAP activity. The results are presented as a percentage of enzyme activity (luminescence) in cells expressing SEAP alone. The results are means±S.E.M. for three independent experiments. (B) COS-7 cells were co-transfected with the SEAP vector and EGFP-N1, CD8, FLAG–hTRPC1, EGFP–TRPC3 or FLAG–hTRPC7 and grown 48 h prior to assaying for SEAP activity. Values are presented as a percentage of the total enzyme activity (luminescence) in the cells expressing EGFP. The results are means±S.E.M. for three independent experiments.
