HSVECs were isolated from CAD patients with (CAD T2D, n=5) and without (CAD ND, n=5) T2D and eNOS Ser¹¹⁷⁷ phosphorylation in the presence or absence of AICAR assessed by Western blotting of cell lysates. (A) Representative immunoblots from two CAD patients with and two CAD patients without T2D. (B) Densitometric analysis of the P-eNOS (phospho-eNOS Ser¹¹⁷⁷)/eNOS ratio relative to an internal standard lysate.*P<0.05 compared with CAD T2D (vehicle).