(A) Representative photomicrographs showing HSVECs in primary culture. Endothelial cells were successfully isolated, as determined by their characteristic cobblestone morphology (left-hand panel) and positive staining (green) for vWF (middle and right-hand panels). (B) HSVEC mitochondrial H2O2 production was determined using the mitochondria-targeted MS probe, MitoB, in both the presence and absence of the mitochondria-targeted antioxidant, MitoQ10. *P<0.05 compared with CAD ND. CAD ND, CAD patients without T2D (n=4); CAD T2D, CAD patients with T2D (n=4); controls, control subjects (n=3).