The main events in vesicular transport of cargo are shown. Cargo is selected and packed into vesicles which are formed by coat proteins (1 and 2). The GTPase Rab is also incorporated on the outside of the vesicle and facilitates the steps illustrated. The vesicle then travels along proteins of the cytoskeleton towards its destination following dissociation of coat proteins (3). The vesicle is tethered to the donor membrane with the help of tethering proteins and the SNARE complexes (4), allowing membrane fusion and release of the cargo (5).