Figure 1
For this cholesterol efflux assay, 100 μl of ILG suspension was mixed with 150 μl of Buffer A in multiple tubes and kept in the dark at 4°C. At various time points (0, 2, 4, 6, 10, 17, 23, 30, and 60 days), 90 μl of supernatant from three tubes was divided into new tubes and kept in the dark at 4°C. After 60 days in storage, the fluorescence (Em: 485 nm, Ex: 538 nm) of 75 μl of the supernatant was simultaneously measured in triplicate. White circles indicate the CEC of 50 μg protein/ml HDL at the start of experiment; AU, arbitrary unit.
Stability of immobilized liposomes in Sephacryl S-300 gel (ILG)

For this cholesterol efflux assay, 100 μl of ILG suspension was mixed with 150 μl of Buffer A in multiple tubes and kept in the dark at 4°C. At various time points (0, 2, 4, 6, 10, 17, 23, 30, and 60 days), 90 μl of supernatant from three tubes was divided into new tubes and kept in the dark at 4°C. After 60 days in storage, the fluorescence (Em: 485 nm, Ex: 538 nm) of 75 μl of the supernatant was simultaneously measured in triplicate. White circles indicate the CEC of 50 μg protein/ml HDL at the start of experiment; AU, arbitrary unit.

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