(A) Eighteen hours of LRRK2in1 treatment in the presence and absence of torin-1 to block m-TOR and induce de-phosphorylation of Ser758 ULK1 and Thr389 P70S6K. The gel shown is representative of three independent experiments, variation of pSer758-ULK1/total ULK1 (mean and standard deviation) was quantified for each treatment versus the control in DMSO (DMSO control = baseline, set to 1). Statistical analysis was performed by one-way ANOVA followed by Tukey’s post-hoc test. (B) Eighteen hours of LRRK2in1 treatment after 4 h inhibition of m-TOR achieved by torin-1 treatment. The gel shown is representative of three independent experiments, variation of pSer758-ULK1/total ULK1 (mean and standard deviation) was quantified for each treatment versus the control in DMSO. Statistical analysis was performed by one-way ANOVA followed by Tukey’s post-hoc test; **P<0.01 and ***P<0.001.