Expression patterns of several genes related to basic functions of GCs from different sized follicles during in vitro culture
(A) and (B) represent expression of proliferation, apoptosis genes, and expression of steroidogenesis genes mRNA respectively. Bars with different lowercase latter are significantly different for the same type of cell during the cultured time in vitro (P<0.05). * indicates significant differences among GCs of three different stages in the same cultured time (P<0.05 and P<0.01 respectively). GAPDH and β-actin were used as internal controls.
“F1 GCs” represents the GCs from the largest follicle (F1) and “F4–F2 GCs” represents the GCs from the fourth to the second largest follicles (F4–F2).