Digestion of GCs from follicles at different developmental stages
The concentrations of collagenase are 0.1% and 0.3%, and the morphological differences among three cohorts of GCs cultured in vitro were observed under a microscope (×100). The rectangular box indicates the granulosa layer without being fully digested.
“F1 GCs” represents the GCs from the largest follicle (F1) and “F4–F2 GCs” represents the GCs from the fourth to the second largest follicles (F4–F2).