Effects of AMPK on mTOR signaling in the failing hearts
Mice were first subjected to AB for 4 weeks to induce chronic heart failure, followed by intraperitoneal injection of 0.5 mg/g AICAR (AMPK activator) for another 4 weeks. Representative immunoblots (A) and the related quantitative analysis (B,C) of phosphorylated and total 4EBP1, p70S6K, and AKT expression in heart failure. *P<0.05 compared with the corresponding sham or vehicle control group; #P<0.05 compared with the sham + NS group; $P<0.05 compared with the AB + NS group (the blots represent four independent experiments, n=4).