Dose-dependent promotion of osteoblast migration by rhHMGB1 without inhibiting cell viability
(A) Osteoblasts were seeded into the upper chamber of the transwell, treated with rhHMGB1, and allowed to invade the matrigel for 4 h. The invasive cells that migrated through the basal membrane to the lower surface were stained with crystal violet, and were photographed at 10× magnification. (B) Migrated cells were counted under a light microscope in five randomly selected microscopic fields. One-way ANOVA was performed to determine statistical significance. rhHMGB1 promotes osteoblast migration in a dose-dependent manner (**P<0.01), which reached a peak at 150 μg/l. (C) Proliferation assay after incubation with rhHMGB1 for 24–72 h. Results of the MTT assay showed that rhHMGB1 had no significant effect on the cellular proliferation of osteoblasts.