Histograms illustrate the quantitative evaluation of BrdU and DCX in the DG (A) and SVZ (B) from WT and Abca7^−/− (A7^−/−) mice. Measurement of the positive BrdU and DCX immunoreactive area in specified regions of interest of both the DG and SVZ was undertaken on five sections for each region in each mouse as described in the “Materials and Methods” section. The staining analysis was quantified by ImageJ software. The number of mice analysed was WT (n=7) and A7^−/− (n=7). There were no statistical differences in the area (μm²/section) for BrdU or DCX immunoreactivity in either the DG or SVZ when WT and Abca7^−/− animals were compared. Data (mean±S.E.M.) in histograms represent the relative value as normalized with to WT (arbitrarily set at 1.0).