Representative coronal sections immunostained for BrdU and DCX quantification in the DG
Low-magnification images (selected from −1.8 to −2.2 mm posterior to Bregma from an 8-month-old male WT mouse) demonstrate the distribution of BrdU (A) and DCX (D) immunoreactivity in a representative coronal sections. As predicted, the majority of BrdU +ve and DCX +ve cells were located in the hippocampus. Panels “B” and “E” are enlarged photomicrographs of the boxed areas from panels “A” (BrdU) and “D” (DCX) respectively. Panels “C” and “F” are high-power images illustrating BrdU and DCX DG staining patterns in more detail. The broken-line arrows point to the corresponding zoomed pictures. Scale bar: for A and D is 500 μm; for B and E is 150 μm; for C and F is 25 μm).