Figure 1
Binding isotherms of rAtACBP6 titrated with (A) palmitoyl-CoA (C16:0-CoA), (B) oleoyl-CoA (C18:1-CoA), (C) linoleoyl-CoA (C18:2-CoA) and (D) linolenoyl-CoA (C18:3-CoA) at 30°C
Top panels show the raw data from 20 injections of 1.8-μl aliquots of a 0.25 mM (A, C and D) or 0.5 mM (B) solution of acyl-CoA into a cell containing 0.015 mM rAtACBP6 at 30°C; bottom panels show the integrated area of each injection after background correction.
