Representative autoradiograph and gel stained with Coomassie Brilliant Blue are shown, band shifts obvious for p110 are because of the presence or absence of His-tag; Quantitation of His-tag (solid bars) and His-tag-free (open bars) kinases as labelled for phosphorylation of (A) p110, B) p85 (all isoforms except p110γ) and (C) βic (median ± S.E.M., n≥2), asterisks indicate the statistical significance as shown **P<0.01, *P<0.05. Autoradiography units corrected for protein loading based on Coomassie Brilliant Blue staining intensity.