Figure 3
Representative autoradiograph and quantitation; His-tag (solid bars) and His-tag-free (open bars) kinases as labelled (median±S.E.M., n≥3).
Effects of His-tag on lipid kinase activity as measured by generation of PI(3)P in the radioactive TLC assay

Representative autoradiograph and quantitation; His-tag (solid bars) and His-tag-free (open bars) kinases as labelled (median±S.E.M., n≥3).

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