Figure 3
![HT1080 fibroblasts and SH-SY5Y neurons were grown to ~80% confluence in 6-well plates and metabolically labelled with [14C] propionate under the standard culture conditions or in the presence of either chloroquine (25 μm) or leupeptin (40 μm) for 48 h. The cells were then subjected to 5% TCA and [14C] propionate in the cell pellets determined and expressed as a percentage of the control conditions (A). Pearson's correlation analysis was conducted to assess potential associations between changes in relative mitochondrial [57Co]Cbl levels (derived from the experiments shown in Figures 1 and 2) and [14C] propionate incorporation into TCA-precipitated cell pellets (B).*P<0.05, #P=0.07.](https://port.silverchair-cdn.com/port/content_public/journal/bioscirep/34/1/10.1042_bsr20130130/2/bsr034e092fig3.jpeg?Expires=1716269365&Signature=EkqyP1k-xLFnZ~RrTENBo10-2sNwz4z~vJR3XxKDsXIDdF89JS3QS75RdP7D8dOywLfawyUYKWMgUhBA2V~PSO0ss331ZtpiWrbqiizXbNSlUN7OhwOzu3qOaNc3w04JtCTEupzUZerJ3YCXmNcEW4aF~DGQjo2oNHGFxODxg7welqCqlBGec0iV-22KDs~zEo3KjRLCsc1j2dVnHPdAc0Y2J7zZle323Awa1XYz5So3ynS42nVzYaEigvJgvhTy-zIhYkG81EJ9sHl2XBO6l2nhsKhoC4X2NzHwegiDL6eQ0HBXGXvEFQ8sAsPiu4LiviFjmoABYgjJnwZR20vHnA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Protease inhibitors reduce cellular [14C] propionate incorporation
HT1080 fibroblasts and SH-SY5Y neurons were grown to ~80% confluence in 6-well plates and metabolically labelled with [14C] propionate under the standard culture conditions or in the presence of either chloroquine (25 μm) or leupeptin (40 μm) for 48 h. The cells were then subjected to 5% TCA and [14C] propionate in the cell pellets determined and expressed as a percentage of the control conditions (A). Pearson's correlation analysis was conducted to assess potential associations between changes in relative mitochondrial [57Co]Cbl levels (derived from the experiments shown in Figures 1 and 2) and [14C] propionate incorporation into TCA-precipitated cell pellets (B).*P<0.05, #P=0.07.
