Figure 3
Ca2+ transients induced by AVP after pre-treatment with thapsigargin in hyperglycaemic and normal glucose-cultured A7r5 cells
For both (A) NG and (B) HG culture conditions, the main Figures show results from four coverslips, whereby each trace represents averaged fluorescence values from ten cells, analysed for Fluo-3 fluorescence changes (F/F0) in response to 10 nM AVP, after pre-treatment of cells with thapsigargin. The insets show the typical response profile (averaged from ten cells) for 10 nM AVP after pre-treatment with DMSO (vehicle control for thapsigargin) in cells from the same glucose culture conditions. Arrows indicate when AVP was added.
