Comparison of gene transduction by PTD.AdeGFP and control virus AdeGFP
(A) PTD.AdeGFP-infected A549 cells compared with unmodified control virus AdeGFP in the presence of soluble fibre knob (with soluble fiber knob) (5 μg/ml) or absence of soluble fibre knob (w/o soluble fiber knob). (B) Comparison of gene transduction by PTD.AdeGFP and AdeGFP in CHO–CAR or CHO cells. (C) Comparison of gene transduction by PTD.AdeGFP and AdeGFP in different cell lines. Representative areas were imaged at ×20 magnification, except for C39 and HUVEC cells (×10 magnification) using an Olympus IX70 microscope. (D) Comparison of gene transduction by PTD.AdeGFP and AdeGFP in CAR-deficient cells derived from different tissues. (E) Effect of the presence or absence of the Tat PTD peptide on PTD.AdeGFP transduction in CHO cells. In (A, B, D and E), results are means±S.D. (n=3). Statistical significance was determined by the Student's t test. 3T3, NIH 3T3.