PTD.AdeGFP contains the eGFP gene in the E1 region under the control of the RSV promoter. The pBS shuttle vector containing the Tat PTD motif in the HI loop of Ad5 fibre linearized by BamHI and EcoRI was co-transformed into E. coli. BJ5183 cells along with SwaI linearized pTG360RSVeGFP/SwaI. The positive clones were screened by enzyme digestion and DNA sequencing. Appropriate recombinants were digested by PacI and transfected into HEK-293 cells. Viruses were harvested, amplified and purified using standard methods. Gluc, glucuronidase.