(A) top panels: 8 weeks of HFD feeding stained with Oil Red O (n=3) versus bottom panel: 12 weeks HFD with single trodusquemine injection given at week 8 HFD (n=3); (B) quantification of plaque area as analaysed using Image J software. Data are represented as mean +/- S.E.M. and analysed by unpaired two-tailed t test where ** p=0.0014.