Prorenin/renin was measured in the liver and kidney tissues from wild-type (WT) and transgenic rats (TG) fed with I3C (+) or without I3C (−) by RT-PCR (n=5/each group) (A), in plasma (n=5/each group) (B), renal cortex tissue (n=5/each group) (D), and urine samples (n=5/each group) (E) by Western blot assay. Further, 24 h after BNX, plasma prorenin/renin levels were determined by Western blot assay (C) (n=3/each group). β-actin served as control.