Figure 5
(A) (Fibroblastic focus): aggregated αSMA-positive, LTBP2-negative, and lineage-negative myofibroblasts (arrows) and αSMA-negative, LTBP2-negative, and lineage (CD324)-positive epithelial cells (triangles). (B) (Fibrotic interstitium): a non-aggregated αSMA-positive, LTBP2-positive, and lineage-negative myofibroblast (arrow). (C) (Fibrotic interstitium): αSMA-positive, LTBP2-negative, and lineage (h-caldesmon)-positive smooth muscle cells at an alveolar wall (arrows). (D) (Bronchiole): αSMA-positive, LTBP2-negative, and lineage (h-caldesmon)-positive smooth muscle cells around a bronchiole (arrows); αSMA-negative, LTBP2-negative, and lineage (CD45)-positive alveolar macrophages (triangles). (E) (Blood vessel) αSMA-negative, LTBP2-negative, and lineage (CD31)-positive vascular endothelial cells (arrows). (F) (Lymphatic vessel): αSMA-negative, LTBP2-negative, and lineage (podoplanin)-positive lymphatic endothelial cells (arrows). All scale bars = 10 μm.
Representative immunofluorescence images of a biopsied lung of IPF (color: green = αSMA; red = LTBP2; white = lineage markers (CD45, CD324, CD31, podoplanin, and h-caldesmon))

(A) (Fibroblastic focus): aggregated αSMA-positive, LTBP2-negative, and lineage-negative myofibroblasts (arrows) and αSMA-negative, LTBP2-negative, and lineage (CD324)-positive epithelial cells (triangles). (B) (Fibrotic interstitium): a non-aggregated αSMA-positive, LTBP2-positive, and lineage-negative myofibroblast (arrow). (C) (Fibrotic interstitium): αSMA-positive, LTBP2-negative, and lineage (h-caldesmon)-positive smooth muscle cells at an alveolar wall (arrows). (D) (Bronchiole): αSMA-positive, LTBP2-negative, and lineage (h-caldesmon)-positive smooth muscle cells around a bronchiole (arrows); αSMA-negative, LTBP2-negative, and lineage (CD45)-positive alveolar macrophages (triangles). (E) (Blood vessel) αSMA-negative, LTBP2-negative, and lineage (CD31)-positive vascular endothelial cells (arrows). (F) (Lymphatic vessel): αSMA-negative, LTBP2-negative, and lineage (podoplanin)-positive lymphatic endothelial cells (arrows). All scale bars = 10 μm.

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