(A) Representative images of FACS analysis for steady-state fibroblasts (FB) from untreated mice (UM) and activated FB/myofibroblasts (MFB) from bleomycin-treated mice (BM). (B) Fluorescence intensities of FITC-conjugated anti-αSMA antibody in steady-state FB, activated FB, and MFB (n=4 per group). Statistical comparisons by t test were performed using the value of steady-state FB as a control. *P<0.05. (C) Heatmap generated by microarray analyses. Genes up-regulated in MFB were selected according to the following criteria: (i) five-fold greater than in steady-state FB; (ii) five-fold greater than in total lung homogenates; and (iii) two-fold greater than the mean value of all genes in MFB. Conversely, genes up-regulated in steady-state FB were defined according to the following criteria: (i) five-fold greater than in MFB; (ii) five-fold greater than in total lung homogenates; and (iii) two-fold greater than the mean value of all genes in steady-state FB. The listed genes are the top 20 genes with high signals in each group and are available in the NCBI Reference Sequence.