IL-1β-induced FGF-2 expression promotes EPC migration and tube formation
ATDC5 cells were incubated with IL-1β at different dosages for 24 h, pre-treated with FGF-2 NAb for 30 min before incubation with IL-1β for 24 h, or incubated with IL-1β for the indicated times. The CM was collected, and then applied to EPCs. An EPC migration assay (A, B, E and F; n≥6) and tube formation assay were performed (C and D; n=8); Scale bar, 200 μm. Quantification results are expressed as means±S.D. *P<0.05 compared with the Con group (control) in (B) and (D), and compared with the 0 h group in (F); #P<0.05 compared with the IL-1β-treated group in (B) and (D), and compared with the 36 h group in (F).