IL-1β induces FGF-2 expression via IL-1RI in chondrocytes
ATDC5 cells were incubated with IL-1β in different dosages for 24 h, or with IL-1β for the indicated times. FGF-2 expression was examined by Western blotting (A and C; n≥6) and ELISA (B and D; n≥6). ATDC5 cells were pre-treated with IL-1RI NAb for 30 min, and then stimulated with IL-1β for 24 h, after which FGF-2 expression was examined by Western blotting (E; n=5) and ELISA (F; n=6). Quantification results are expressed as means±S.D. *P<0.05 compared with the Con group (control) in (B) and (F), and compared with the 0 h group in (D); #P<0.05 compared with the IL-1β-treated group in (F). Molecular masses are indicated in kDa. β-Actin was used as a loading control.