APCs from naive mice were co-cultured with CFSE-labelled CD3⁺ T-cells from DO11.10 mice in the presence of pOVA. Pooled CD3⁺ T-cells from pLNs isolated 24 h after injury or sham treatment (n=3 mice per group) were added to the co-cultures at various ratios, as indicated. CFSE-labelled CD3⁺ T-cells alone served as a negative control. (A) Representative histograms of CFSE dilution after 3 days of culture. The numbers indicate the percentage of proliferating DO11.10 cells. Dashed lines separate proliferating and non-proliferating cells. (B) Concentration of IFN-γ in the supernatants. Data show mean ± S.D. of triplicate cultures. Data on proliferation and IFN-γ secretion are representative of five independent experiments.