Role of endogenous T-cells in injury-induced Th1-cell suppression
Wt BALB/c mice RAG2−/− mice or RAG2−/− mice that had received purified CD3+ T-cells from naive BALB/c mice 24 h earlier underwent injury or sham treatment. CFSE-labelled T-cells from DO11.10 mice were applied, OVA protein was injected into the footpads and pLN cells were pooled per group (n=3 mice each). (A) Overview of the experimental design. (B) Representative histograms of CFSE dilution in gated OVA-specific Th-cells of pLN cells. (C) The pLN cells were restimulated with pOVA and the concentration of IFN-γ was determined. Data show mean ± S.D. of triplicate cultures (n=3 mice per group) and are representative of three independent experiments. Statistically significant differences between sham-treated mice and injured mice were detected with Student's t-test. **P<0.01; ***P<0.001.