Inverse capability of exogenously loaded DCs and endogenous APCs to Th1-cell priming in vivo
OVA protein or BMDCs previously loaded with OVA in vitro were injected into the hind footpads 4 days after injury or sham treatment and after transfer of CFSE-labelled OVA-specific Th-cells. After 3 days, pLN cells were pooled per group. (A) Overview of the experimental design. (B) Percentage of CD25+ and CD69+ cells in gated OVA-specific Th-cells. (C) Content of IFN-γ in the supernatants after restimulation of pLN cells with pOVA. Data show mean ± S.D. of triplicate cultures and are representative of three independent experiments with n=3 mice per group. Statistically significant differences were detected with Student's t-test. *P<0.05, sham treatment compared with injury.