(A) Mice were infected with SP on day 0 and IAV on day 1 as described in Figure 1A. On days 4 and 6, mice were treated intranasally with AT-RvD1 (SPIAV-RvD1) or vehicle (SPIAV-Veh) once daily. On day 7, mice were culled and outcomes were measured. (B) Freshly collected BALF was serially diluted and incubated on horse blood agar supplemented with 5 µg/ml gentamicin overnight at 37°C and CFUs were recorded from highest countable dilution. (C) Lung tissue that had been snap-frozen was homogenized and microbial DNA qPCR was carried out to quantitate SP levels. (D) qPCR on viral RNA extracted from lung tissue was performed to quantitate IAV levels. (E) Taqman qPCR was performed on lung mRNA extraction for assessment of IFNγ gene expression. (F) CD8⁺ T cells in the lungs were determined by flow cytometry, demonstrating an increase in CD8⁺ numbers in coinfected mice that was not altered by AT-RvD1. n=5–12, *P<0.05, two-tailed Student’s t tests.