Characterization of BAL inflammation after acute smoke exposure reveals lack of neutrophilic influx in sidestream CS-exposed mice
The lungs of mice exposed to CS for 3 days were lavaged with 4×0.5 ml aliquots of sterile PBS. Total cell counts were determined in a haemocytometer via Trypan Blue exclusion. Differential cell counts were performed using morphological criteria on May–Grünwald-Giemsa–stained cytospins (200 cells/sample). (A) Total cell counts. (B) Macrophages. (C) Neutrophils. (D) Lymphocytes. Results are means±S.D., one-way ANOVA following Bonferroni post-hoc test. **P<0.01 and ***P<0.001; FA, filter air; n=12. ns, not significant.