Using MitoB to assess mitochondrial hydrogen peroxide formation in vivo.
Administration of MitoB to an animal model leads to the rapid accumulation of MitoB within cells and from there into mitochondria, driven by the plasma (Δψp) and mitochondrial (Δψm) membrane potentials. There MitoB will react with the local concentration of hydrogen peroxide to form MitoP causing the ratio of MitoP/MitoB to increase.