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Figure 2

$(A) β-Catenin was assessed by western blot in the cytoplasmic and nuclear fractions of HT29 cells (1×106 cells) cultured alone (HT) or in the presence of platelets (+PLT), up to 20 h; quantification of attenuance (D) of different specific bands was calculated using laser densitometry and normalized to the D of β-actin and values were reported; lamin A and C, which are essential scaffolding components of the nuclear envelope, expression was detected in the same samples by Western blot technique.(B) Wnt3a levels were assessed by western blot in supernatants of HT29 cells cultured alone or with platelets.$

Effect of platelets on β-catenin translocation into the nucleus

(A) β-Catenin was assessed by western blot in the cytoplasmic and nuclear fractions of HT29 cells (1×10⁶ cells) cultured alone (HT) or in the presence of platelets (+PLT), up to 20 h; quantification of attenuance (D) of different specific bands was calculated using laser densitometry and normalized to the D of β-actin and values were reported; lamin A and C, which are essential scaffolding components of the nuclear envelope, expression was detected in the same samples by Western blot technique.(B) Wnt3a levels were assessed by western blot in supernatants of HT29 cells cultured alone or with platelets.

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