(A) Cell migration assay. HeLa cells were transfected with HOTAIR siRNA or scramble and the cell monolayers were wounded by scraping with a pipette tip. The cell migrations were monitored at 0 and 24 h. (B) Cell migration distances were measured by ImageJ software. (C) Matrigel invasion assay. (D) Quantitation of (C). (E) HeLa cells were transfected with HOTAIR siRNA or scramble for 24 h, the protein levels of MMP-9 and VEGF were assayed by Western blot. (F,G) Quantitation of (E). All the experiments were repeated at least three times and representative results were shown. GAPDH was used as loading control; **P<0.01, ***P<0.001 compared with scramble.