HOTAIR knockdown increases apoptosis in cervical cancer cells
(A) HeLa cells were transfected with HOTAIR siRNA or scramble for 24 h, the mRNA levels of HOTAIR were measured by qRT-PCR. (B) HeLa cells were transfected with HOTAIR siRNA or scramble for 24, 48, 72, and 96 h, the cell viability was assayed by CCK-8 kit. (C) The cell apoptosis was analyzed by Annexin V flow cytometry. (D) Apoptotic cell quantitation for three independent experiments. (E) Protein levels of Ki67 and PCNA were assayed by Western blot. (F) Protein levels of cleaved caspase-3 and cleaved caspase-9 were assayed by Western blot. All the experiments were repeated at least three times and the representative data were shown. GAPDH was used as loading control; *P<0.05, **P<0.01 compared with scramble.