Effect of cytochalasin D treatment on ECM patterning of different TSP family members
(A) Representative fluorescence images of ECM deposition patterns of V5-tagged TSP1, Drosophila TSP (D-TSP), TSP5 or Ciona TSPA (Ci-TSPA) after treatment of cells with vehicle (DMSO) or 0.5 μM cytochalasin D for 18 h. An image of TSP1-V5 without primary antibody is included as a negative control. (B) Quantification of V5-expressing cells for each protein under the different experimental conditions. (C) Quantification of edge-concentrated ECM puncta for each protein under the different experimental conditions. (B and C) Columns represent the mean and bars indicate S.E.M. from three independent experiments. *P<0.05, **P<0.01, ***P<0.001.