(A) Representative fluorescence images of ECM deposition patterns of mRFPovTSP1C after treatment of cells with vehicle (DMSO), 0.5 μM cytochalasin D, 0.67 μM jasplakinolide or 0.5 μM latrunculin B for 18 h. (B) Quantification of mRFPovTSP1C-expressing cells under the different experimental conditions. (C) Quantification of edge-concentrated ECM puncta under the different experimental conditions. (B and C) Columns represent the mean and bars indicate S.E.M. from three independent experiments. ***P<0.001.