(A) Western blotting with anti-tubulin antibody (in 1:5000 dilution) to show a similar amount of each lysate was loaded. (B) A representative MADA result showing DNase II enzymatic activity. (C) Quantitative analysis was determined by the area of the clear zone on agarose from three independent experiments. The enzymatic activity of DNase II in N2 is served as 100% and the relative percentage of DNase II activity from each strain of worms is indicated. N=3, Error bars indicate S.E.M., *P > 0.05, **P > 0.01, ***P > 0.001.