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Figure 4

$Analytical ultracentrifuge analysis showing the integral distribution of the standard sedimentation coefficients (s20,w) of a histone H1-depleted chromatin fraction reconstituted with recombinant H1.1 (WT) or H1.1 consisting of swapped NTD and CTD (SW) at different ionic strength conditions: 5 mM NaCl, 10 mM Tris/HCl (pH 7.5) 0.1 mM EDTA (A) or 80 mM NaCl, 10 mM Tris/HCl (pH 7.5) 0.1 mM EDTA (B). The insert in (A) shows an SDS/PAGE of the histone composition of the samples used in the analysis at a ratio of 1.0 or 1.5 (mol H1, mol nucleosome).$

Histone H1.1-dependent chromatin folding

Analytical ultracentrifuge analysis showing the integral distribution of the standard sedimentation coefficients (s_20,w) of a histone H1-depleted chromatin fraction reconstituted with recombinant H1.1 (WT) or H1.1 consisting of swapped NTD and CTD (SW) at different ionic strength conditions: 5 mM NaCl, 10 mM Tris/HCl (pH 7.5) 0.1 mM EDTA (A) or 80 mM NaCl, 10 mM Tris/HCl (pH 7.5) 0.1 mM EDTA (B). The insert in (A) shows an SDS/PAGE of the histone composition of the samples used in the analysis at a ratio of 1.0 or 1.5 (mol H1, mol nucleosome).

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