Figure 2
Position-dependent ubiquitination is required for Pex5p function
Mutant pex5Δ cells were transformed with plasmids expressing Pex5p or indicated variants. (A) Immunoblot analysis of equal amounts of whole-cell trichloroacetic acid lysates of indicated strains with antibodies against Pex5p. Detection of mitochondrial porin served as loading control. (B) Indicated strains were spotted as a series of 10-fold dilutions on a medium with oleic acid as sole carbon source and incubated for 4 days at 30°C. Growth of mutant pex5Δ cells expressing Pex5p or Pex5pK18C was indistinguishable from the wild-type, whereas the mutant pex5Δ cells expressing Pex5pC6A/K18C display no growth on this carbon source.
