Transcriptional activity of Tfam promoter truncations and effect of AMPK activation
(A) The responsiveness of the various promoter-luciferase reporter constructs to differentiation-induced activation was assessed on days 0, 1, 2, 3 and 4 (n=7). *P<0.05, −1706 compared with −2051 kb, −978 kb compared with −2051 kb, −393 compared with −2051 kb. ¶P<0.05, −978 kb compared with −1706 kb, −393 kb compared with −1706 kb, −78 kb compared with −1706 kb. (B) C2C12 cells were treated with AICAR (1 mM) or vehicle for 24 h. Representative western blot probed with Phospho-AMPKα (Thr172). Summary of the effect of AICAR on AMPK activation is shown (n=3). (C) The relative luciferase activity of the Tfam promoter constructs in vehicle- or AICAR-treated cells (n=7). *P<0.05, AICAR compared with vehicle. Values are expressed as means±S.E.M.