Protein expression of mitochondrial complexes normalized to vinculin (Vinc) loading control in heart homogenate from 14-week-old mice fed ad libitum (L) and after a 4 week 40% calorie restriction (R). Quantification of complex I (CI; A), II (CII; B), III (CIII; C), IV (CIV; D) and V (CV; E) expression and representative Western blot (F). Values are mean ± S.E.M., n = 8. Black=C (control offspring), white=U (in utero undernourished offspring).