(A), (B) and (C) Representative gels of at least three independent biological experiments showing EXO1 catalysing forked, gap and nick DNA substrates with a 5′ flap of (A) 1 nt, (B) 5 nt and (C) 10 nt. (D) Statistical analysis of degradation of fork, gap and nick substrate of different length by EXO1. One-way analysis of variance with Tukey's multiple-comparison test with significance. The letters indicate F=fork, G=gap and N=nick substrate, ns=not significant. Stars in the table indicated the significance (P<0.05) between the different parameters.