The cellular pharmacokinetic profile of hypericin in RINm5F insulinoma cells
Representative live-cell confocal images (rows 2 and 4) and corresponding transmission images (rows 1 and 3) were acquired at indicated time points from cells exposed to 100 nM hypericin. Hypericin fluorescence became detectable in the plasma membrane and sub-plasma membrane region within 20 min and then appeared in the cytoplasm. Hypericin fluorescence in cells reached its maximum brightness in ~1 h. The experiments were repeated six times. Bar=10 μm.